Resources

Tierra_AppNote-ProteinQuant02.pdf

App Note

The vast diversity of proteins poses challenges for high-throughput analytical approaches, as certain proteins may exhibit compatibility issues, leading to inaccurate readings. We have pinpointed specific trends in protein sample properties that significantly impact the accuracy and consistency of protein quantification outcomes. We used these insights to generate key considerations and best practices for setting up high-throughput protein characterization workflows.

Challenging proteins: Lessons learned from a large protein dataset

Tierra_AppNote-ProteinQuant01.pdf

Despite the complex requirements for protein characterization, such as precision, sensitivity, and versatility, currently, no single analytical method can fulfill all these demands simultaneously for high throughput applications. We developed a synergistic workflow that integrates UV/Vis spectrophotometry with microfluidicsbased capillary electrophoresis to streamline the high throughput characterization of diverse proteins.

Resources

Challenging proteins: Lessons learned from a large protein dataset

Considerations and best practices for high-throughput protein characterization workflows

Challenging proteins: Lessons learned from a large protein dataset

Towards complete protein characterization at scale

An integrated strategy for quality assessments in high-throughput environments.

Towards complete protein characterization at scale